Acta Pharmacologica Sinica 2005 Jun; 26 (6): 721-728; doi: 10.1111/j.1745-7254.2005.00111.x

Aim: To investigate the preventive and protective effects of bendazac lysine (BDL) on experimental early diabetic nephropathy (DN) rats.

Methods: After an early DN model was induced by streptozotocin, rats were administered BDL at doses of 100, 200, and 400 mg/kg for 8 weeks. Blood glucose, microalbuminuria, kidney index, total antioxidative capacity, laminin, advanced glycation end pro-ducts (AGE), aldose reductase (AR) activity, and the relative quantity of transforming growth factor b1 (TGF-β1) mRNA were measured by different methods. The ultrastructural morphology was observed by transmission electron microscope.

Results: The physical behaviors of early DN rats were hypopraxia, cachexia, and polyuria, while those treated with high doses of BDL were vibrant and vigorous. For BDL-treated DN rats, when compared with vehicle-treated DN rats, the blood glucose level and the intensity of oxidative stress were ameliorated. Also, the microalbuminuria level, AGE either in serum or in renal, and AR activity were significantly reduced. Furthermore, the expression of TGF-β1 mRNA in the kidney cortex was declined and the thickness of glomerular base membrane was decreased significantly. The ultrastructure of glomerulus and mesangial matrix of BDL-treated DN rats were ameliorated.

Conclusion: BDL has protective effects on several pharmacological targets in the progress of DN and is a potential drug for the prevention of early DN.

Keywords: bendazac lysine; diabetic nephropathies; blood glucose; oxidative stress