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Acta Pharmacologica Sinica 2005 November; 26 (11): 1395-1401; doi: 10.1111/j.1745-7254.2005.00188.x |
| Original Article | [ Full text ] |
| Multivesicular liposome formulations for the sustained delivery of interferon α-2b1 |
Jian QIU1, Xiao-hui WEI1, Fang GENG1, Rui LIU1, Jing-wu ZHANG2, Yu-hong XU1,3 1School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200030, China; 2Joint Immunology Laboratory of Institute of Health Science and Shanghai Institute of Immunology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Second Medical University, Shanghai 200025, China |
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Aim: To develop and optimize a sustained release multivesicular liposome (MVL) formulation of interferon (IFN) α-2b. Methods: Methods: IFN α-2b MVL were prepared using a typical double-emulsion procedure. The sustained release effects of IFN α-2b MVL were investigated by monitoring the blood IFN α-2b concentration using an enzyme-linked immunosorbent assay test after subcutaneous administration to healthy mice. Results: IFN α-2b was successfully encapsulated in MVL with high efficiency, and the integrity of encapsulated protein was maintained. After subcutaneous injection, the MVL slowly released IFN α-2b into systemic circulation in a sustained manner. The estimated serum half-life of IFN α-2b was approximately 30 h. In addition, varying the size of the MVL preparations could further modify the in vivo release profile.
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Keywords: interferon α-2b; liposomes; delayed-action preparations |
| 1 Project supported by Science and Techno-logy Commission of Shanghai Municipality (No 024319120 and No 04DZ14902). |
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