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Acta Pharmacologica Sinica 2005 November; 26 (11): 1317-1321; doi: 10.1111/j.1745-7254.2005.00178.x |
| Original Article | [ Full text ] |
| Chemokine RANTES is upregulated in monocytes from patients with hyperhomocysteinemia1 |
Wei SUN2,5, Guang WANG3,5, Zhen-min ZHANG4, Xiao-kun ZENG2, Xian WANG2,4,6 2Institute of Vascular Medicine; 3Department of Gerontology, Peking University Third Hospital; 4Department of Physiology and Pathophysiology, Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Basic Medical College, Peking University, Beijing 100083, China |
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Aim: To investigate the changes in plasma level of the chemokine RANTES (regulated upon activation, normal T cells expressed and secreted) and the responsiveness of lipopolysaccharide (LPS)-induced RANTES secretion from monocytes in patients with hyperhomocysteinemia (HHcy). Methods: The plasma levels of homocysteine (Hcy), folate, and RANTES were measured in 38 control patients with normal Hcy levels and 40 patients with HHcy and the mRNA synthesis of RANTES in isolated human monocytes was determined by RNase protection assays. Results: The plasma level of RANTES was elevated in HHcy patients compared with controls (median 5.3 vs 3.5 ng/mL, P<0.01). LPS-induced RANTES production from monocytes of HHcy patients was also increased significantly. In addition, Hcy directly increased the mRNA level of RANTES in isolated normal human monocytes in a time- and dose-dependent manner.
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Keywords: homocysteine; monocytes; RANTES; folic acid; atherosclerosis; humans |
| 1 Project supported by the Major National Basic Research Program of China (No G2000056908), Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT), and a grant from the National Natural Science Foundation of China (No 30330250) to Prof Xian WANG. |
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